Kinase Profiling & HTS
Kinase Hotspot SM
Pharma labs typically only use P33 radiolabeled kinase assay profiling for only
a few select compounds. Now, RBC's proprietary nanoliter screening techniques make
this gold standard kinase assay format available for large-scale profiling and HTS.
Now you can get radioisotope data for a fluorescent price. Read the
Expert Opinion review on the challenge of selecting
protein kinase assays for lead discovery optimization.
Benefits of Kinase HotSpot SM include:
-
Gold Standard radiolabeled data: Avoid the false negatives and positives that are
generated by fluorescent assay formats;
-
440 Kinase assays available, and growing;
-
Costs can be as low as outsourced fluorescent screens;
-
HotSpot's low cost lets you profile more compounds earlier, and avoid dead-end leads;
-
HotSpot HTS brings economic radiolabeled assaying to HTS: Shorten your hit validation
timeline;
-
Flexible ATP concentration from 1µM to 10µM can be used. Low 1µM ATP concentration
means that ATP competitive inhibitors are easier to spot.
Kinase HotSpot SM screens are available
in:
|
Kinase Assay Type
|
Sample Price*
|
|
HTS — 1 kinase
|
$3.00 / well:
20,000 compound order
|
10 dose IC50 vs 50
kinase assay panel
|
$50 / IC50:
20 compounds
|
10 dose IC50 vs 100
kinase assay panel
|
$40 / IC50:
20 compounds
|
10 dose IC50 vs 200
kinase assay panel
|
$30 / IC50:
20 compounds
|
* The price is subject to change and discounts may apply for larger orders. Please
call 1-877-347-2368 or 1-610-722-0247 for details.
Kinase Assay Services Available:
-
Larger scale HTS;
-
Large scale single dose, duplicate profiling;
-
IC50 profiling — 5 or 10 dose with curve fitting;
-
ATP — competitive evaluation;
-
Compound binding reversibility test;
-
Ki determination;
-
Custom Assay development;
-
Substrate determination;
-
Research collaboration.
RBC can quote any subset of its 400 Kinase availability. Pricing is subject to change
without notice. Mutant panel pricing available by quotation only. Custom assay development
for customer-supplied kinases is also available. RBC's nanoliter screening can help
make expensive assays with problem kinases more economical.
For more information on Kinase HotSpot SM,
or for a free price quote, call 1-877-347-2368 or 1-610-722-0247 or email
sales@reactionbiology.com.
HotSpot Image with Resulting Data
|
Z' Factor, c-Src Assay
|
IC50 Values (nM) of Staurosporine Profiled Against 50 Kinases
|
Reproducibility: Actual HTS Run
|
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KINOME
MAPPING APP. DISPLAY YOUR SCREENING RESULTS WITH RBC'S
FREE APPLICATION HERE
A.
Human Kinome Kinases
SPECIAL PRODUCT PANELS:
B.
Receptor Tyrosine Kinases — (also see complete list)
C.
RAF/MEK/ERK Pathway Kinases
— (also see complete list and diagram)
D.
PI3K-AKT Pathway Kinases
— (also see complete list and diagram)
E.
Oncology Kinases—
(also see complete list)
F.
Exploration Kinases—
(also see complete list)
G.
Special Kinases— (also
see complete list)