What’s in YOUR library?
Find out with RBC’s Wide-Field Profiling-Based Discovery

Case Study

An RBC screening partner wanted to find high value hits in an extensive library. RBC applied its Wide-Field Discovery technique as follows:

Step 1 - The library was screened against a gate kinase to find kinase active compounds. 60 hits were found.
Step 2 - The hits were given structure analysis and reduced to 11 compounds.
Step 3 - The 11 selected hits were profiled against 260 kinases.

Result
A potent (<1nM) JAK3 inhibitor was found. At that concentration, the inhibitor was highly selective. After research, it was determined that the scaffold containing this compound had available IP. A few selective inhibitors with low µM range IC50 were also discovered.

Advantage
By being target agnostic, the partner was able to find a high-value compound in its library. By profiling at the beginning of the process, selectivity is assured.

traditional discovery

wide-field discovery

What’s in YOUR library? Find out with RBC’s Wide-Field Profiling-Based Discovery

Step 1 - The library was screened against a gate kinase to find kinase active compounds. 60 hits were found.

Step 2 - The hits were given structure analysis and reduced to 11 compounds.

Step 3 - The 11 selected hits were profiled against 260 kinases.

What’s in YOUR library?
Find out with RBC’s Wide-Field Profiling-Based Discovery