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Reader Domain Proteins

Automated Patch-clamp: QPatch HTX and QPatch 16

The QPatch HTX and QPatch 16 are fully automated patch-clamp platforms that allow for the testing of up to 48 and 16 cells in parallel respectively. These are medium throughput machines great for confirming hits from larger screens and also for lead optimization. The QPatch machines offer high quality measurements with the formation of gigaohm seals. The platform can be used to assay both voltage-gated and ligand-gated channels and utilizes stable cell lines.

Contact us today & discover how our electrophysical assay services can assist you!

  • - Compound profiling against hERG, Nav1.5 and Cav1.2 to evaluate potential cardiac liability
  • - Automated Patch Clamp with QPatch HTX and QPatch 16
  • - Positive control and vehicle control in every assay
  • - Single concentration profiling and full concentration response curves (6 pt. curves; n=3 cells).

Fig. 1: Exemplar hERG trace elicited from a holding potential of -80 mV followed by steps from -60 to +50 mV in 10 mV increments, tail currents were elicited by a step to -50 mV

Fig. 2 a) Activation plot of hERG fitted with a Boltzmann function, V1/2=-19.4 mV, slope 8.7. b) IV curve of hERG activation currents, peak current -10 mV. (n=11 cells)

QPatch:hERG Inhibition by Terfenadine

Fig. 3 a) Exemplar hERG trace showing the concentration dependent inhibition of channel activity by terfenadine. b) Concentration response curve for the inhibition of hERG by terfenadine.

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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