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Reader Domain Proteins







Bromodomain Assay Kit: BromoMELTTM The Broadest Bromodomain Coverage


Test the selectivity of any compound against a wide range of bromodomains using BromoMELT TM, Reaction Biology's new bromodomain assay kit. BromoMELT is a thermal melt stability assay for bromodomain targets. Thermal shift assays measure the thermal stability of a target protein and the increase in protein melting temperature upon the binding of a ligand to the protein. Protein melting is useful for identifying ligands, buffer conditions, cofactors and drugs affecting protein stability.

BromoMELT is available as a service and as a kit and includes 76 bromodomains in total, representing 53 members of bromodomain family.

Contact us today for more information on BromoMELT or make your purchase now!


BromoMELT

  • Extensive coverage of the bromodomain family
  • Test selectivity of any compound within a few hours
  • Available as a service or in kit format
  • Fast and easy to perform in your lab

View BromoMELT data >
View Instruction Manual >
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Bromodomain Mapper

Bromodomain Assay Kit Components
  • 5x Protein stock plate, 15 uL of each protein
  • Reaction buffer, 5x concentrated solution (1 ml)
  • 5000x SYPRO Orange (8 uL)
  • Control compound mix (5 uL)
  • 100% DMSO (10 uL)
  • 1x 384 well qPCR plate
  • 1x clear seal
  • 96 well dispensing guide
  • Optional pre-test:
  • 5x pre-test protein, 30 uL
  • 1x clear seal, small


  • Components required but not supplied
  • Centrifuge with plate compatible baskets
  • 96 well plate
  • Real time PCR/qPCR instrument
  • Multichannel micropipettor
  • 400x Stock(s) of Test Compound in DMSO


BROMODOMAIN ASSAY KIT















With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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