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Reader Domain Proteins

Manual Patch Clamp Assay

Manual patch clamping is the “gold-standard” for the investigation of ion channel activity. In addition to confirming the activity of potential hits from high or medium throughput screens, manual patch-clamping can be used to assess mechanism of action of compounds and to determine the effects of compounds on the biophysical properties of a channel. Both voltage-gated and ligand-gated channels can be tested using manual patch-clamping. This system utilizes stable cell lines or native cells (neurons, cardiac myocytes, etc.).

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  • - Manual patch clamp of the hERG potassium channel to evaluate potential cardiac liability.
  • - Single concentration profiling and full concentration response curves (4 pt. curves; n=3 cells).

Fig. 1 Exemplar hERG trace elicited from a holding potential of -80 mV, followed by an initial step to -50 mV, then steps from -60 to +40 mV in 10 mV increments, tail currents were elicited by a step to -50 mV.

Fig. 2 a) Activation plot of hERG fitted with a Boltzmann function, V1/2=-9.7 mV, slope 11.4. b) IV curve of hERG activation currents, peak current 10 mV. (n=4 cells)

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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