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Reader Domain Proteins

Sodium Ion Channel Assays

Reaction Biology now offers the voltage-gated sodium channel, Nav1.5, as part of our Cardiac Safety Panel. This assay is...

  • Compound profiling against the voltage gated sodium channel Nav1.5 to evaluate potential cardiac liability
  • Automated Patch Clamp with QPatch HTX and QPatch 16
  • Positive control and vehicle control in every assay
  • Single concentration profiling and full concentration response curves (6 pt. curves; n=3 cells).

Contact us today & discover how our electrophysical assay services can assist you!

Figure 1 A/B. Example of Nav1.5 activation currents (A) and Current/Voltage (I/V) relationship (B). Currents were elicited by stepping the voltage from a holding potential -100 mV to + 80 mV in 10 mV steps

Figure 2. (A) Example of inhibition of Nav1.5 currents by the reference compound tetracaine. Peak Nav1.5 currents were elicited by a step change in membrane potential from -100 mV to -30 mV. (B) Resulting time/current plots from QPatch HTX. Currents were normalized to the negative control peak current and plotted as % inhibition (C, below).

Figure 3 A/B. Elicitation of Nav1.5 ‘late’ current (I Na,late) using the recommended CIPA voltage protocol in conjunction with the channel opener veratridine. In B, current traces are expanded to illustrate the veratridine-induced increases in both peak currents and late currents.

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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