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Reader Domain Proteins

Target Engagement Intracellular Kinase Assay (NanoBRET)

Reaction Biology is now offering target engagement assays in collaboration with Promega using the NanoBRETTM technology. The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding of select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tracer, reversibly bound to a NanoLuc® luciferase-kinase fusions in cells. The intracellular target engagement and selectivity are more physiological relevant and fundamental to the pharmacological mechanism of the compounds. While biochemical and biophysical assays identify the kinase inhibitors in vitro, the NanoBRET™ TE assay serves as a great tool to determine the direct interaction of the compounds to the target kinase in cells. Contact us today to discuss your project!

NanoBret Assays Available

ABL1 View Data
ALK4 View Data
Aurora A View Data
Aurora B View Data
Aurora C View Data
DDR1 View Data
DDR2 View Data
EPHA1 View Data
FGFR1 View Data
FGFR2 View Data
FGFR3 View Data
FLT3 View Data
FYN View Data
IRAK3 View Data
IRAK4 View Data
JAK3 View Data
JNK3 View Data
LCK View Data
LYN View Data
MAP3K10 View Data
MAP4K2 View Data
MAP4K3 View Data
MAPK1 View Data
MAPK11 View Data
MAPK14 View Data
MET View Data
MELK View Data
NEK2 View Data
NUAK1 View Data
PAK4 View Data
PRKACA View Data
RET View Data
RIOK2 View Data
RIPK2 View Data
RPS6KA View Data
SIK1 View Data
SIK3 View Data
SRC View Data
TBK1 View Data
TRKA View Data
TRKB View Data
WEE1 View Data
YES1 View Data

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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