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Reader Domain Proteins

Thermal Shift Assays (TSA)

Thermal shift assays measure the thermal stability of a target protein and the increase in protein melting temperature upon the binding of a ligand to the protein. Protein melting is useful for identifying ligands, buffer conditions, cofactors and drugs affecting protein stability. Reaction Biology now offers thermal shift assays for the reader domain proteins, including bromodomains, chromodomains and other methyl-readers.

Instrument Used:

  • BCFX384 (BioRad)

Contact us today to discuss using our thermal shift assays in your project!

Thermal Shift Assays

View our Thermal Shift assays below

thermal shift melt curve
Differential Scanning Fluorimetry of RBC BRD2-Tndm (His) in Presence or Absence of (+)- JQ1. Thermal denaturation of BRDT-Tndm (His) is detected by increased binding and fluorescence of the dye SYPRO Orange. Addition of the BET Bromodomain inhibitor/ligand (+)- JQ1 stabilizes the protein folding and shifts the Tm from 41 to 44 degrees Celsius.

With the largest functional kinase activity assay panel in the industry, RBC provides kinase, epigenetic, and other profiling services to over 750 companies worldwide. RBC specializes in custom assay conditions, high quality reproducible data, and outstanding service.

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