ADRA1A Calcium Flux Agonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2058
GPCR Name
Human Alpha-1A adrenergic receptor
Gene Name
ADRA1A
GPCR Sub Family
Adrenoceptors
Coupling Partner
Gαq
Uniprot Number
P35348 (https://www.uniprot.org/uniprotkb/P35348/entry)
Cell Type
U2OS/ADRA1A/β-arrestin-1&2 stable cell line
Assay Information
Assay Principle
The calcium flux agonist assay measures agonist-triggered, real-time calcium flux via ADRA1A activation in U2OS/ADRA1A/β-arrestin-1&2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Agonist
Assay Readout
Fluorescence
Reference Compound EC50
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference agonist for ADRA1A
