Activity-based DNA-PK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DNA-PK
Enzyme
Human DNA-PK
HGNC
PRKDC
Synonym
HYRC, HYRC1, DNPK1, p460, p350
Scientific Information
P78527 (http://www.uniprot.org/uniprot/P78527)
Assay Properties
Substrates
Peptide substrate, [EPPLSQEAFADLWKK], 20 μM
DNA, 10 μg/ml
ATP, 10 µM
DNA, 10 μg/ml
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PI-103 | 7.2 |
| Wortmannin | 42 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of DNA-PK
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