FES/FPS Kinase Assay Service

Activity-based FES/FPS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
FES

Enzyme
Human FES

HGNC
FES

Synonym
FPS

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.7
PP2 16,000
AG1478 39,000
D-64406 23,000
Scientific Information
P07332 (http://www.uniprot.org/uniprot/P07332)

Screening Location
Malvern, USA

Reference compound IC50 for FES/FPS
Reference compound IC50 for FES/FPS

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