MARK2/PAR-1Ba Kinase Assay Service

Activity-based MARK2/PAR-1Ba kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MARK2/PAR-1Ba

Enzyme
Human MARK2/PAR-1Ba

HGNC
MARK2

Synonym
EMK1, MGC99619, PAR-1Ba, ELKL

Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 1,800
GW 5074 3,300
H-89 2,300
Scientific Information
Q7KZI7-4 (http://www.uniprot.org/uniprot/Q7KZI7-4)

Screening Location
Malvern, USA

Reference compound IC50 for MARK2/PAR-1Ba
Reference compound IC50 for MARK2/PAR-1Ba

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