Activity-based MAST3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MAST3
Enzyme
Human MAST3
HGNC
MAST3
Synonym
Microtubule-associated serine/threonine-protein kinase 3, KIAA0561
Scientific Information
O60307 (http://www.uniprot.org/uniprot/O60307)
Assay Properties
Substrates
Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Staurosporine | 0.3 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of MAST3
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