Activity-based TAOK3/JIK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
TAOK3
Enzyme
Human TAOK3
HGNC
TAOK3
Synonym
DPK, JIK, MAP3K18, TAO kinase 3
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.5 |
| Ro 31-8220 | 200 |
| GW 5074 | 1,040 |
| H-89 | 21,900 |
Scientific Information
Q9H2K8 (http://www.uniprot.org/uniprot/Q9H2K8)
Screening Location
Malvern, USA
Reference compound IC50 for TAOK3/JIK
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