Activity-based PKCepsilon kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCepsilon
Enzyme
Human PKCepsilon
HGNC
PRKCE
Synonym
PKCE, nPKC-epsilon, PKC-epsilon
Scientific Information
Q02156 (http://www.uniprot.org/uniprot/Q02156)
Assay Properties
Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM, Lipid activator,
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 0.21 nM |
| Ro 31-8220 | 0.55 nM |
| GW 5074 | 34.5 µM |
| H-89 | 290 nM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PKCepsilon
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