5-hydroxytryptamine (Serotonin) receptor 5-HT1B Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
5-HT1B Binding
GPCR Target
Human 5-HT1B
Source
Membrane preparation from HEK293 cells stably transfected with human 5-HT1B receptor.
Scientific Information
HTR1B (https://www.uniprot.org/uniprotkb/P28222/entry)
Assay Properties
Protocol
Radioligand filter binding to 5HT1B membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
GR125743, 10 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| GR125743 | 10.1 |
| 8-OH DPAT | >10,000 |
| Ketanserin | >10,000 |
| Naratriptan | 34.8 |
| Serotonin | 77.7 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for 5-HT1B
PDF