APLNR Calcium Flux Antagonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2094
GPCR Name
Human Apelin receptor
Gene Name
APLNR
GPCR Sub Family
Apelin receptors
Coupling Partner
Gαi
Uniprot Number
P35414 (https://www.uniprot.org/uniprotkb/P35414/entry)
Cell Type
HEK293/Gα16 stable cell line transfected with APLNR expression vector
Assay Information
Assay Principle
The calcium flux antagonist assay measures antagonist-mediated inhibition of agonist-triggered, real-time calcium flux via APLNR activation in APLNR transfected HEK293/Gα16 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Antagonist
Assay Readout
Fluorescence
Reference Compound EC50
| Compound | EC50 (nM) |
|---|---|
| ML221 | 1386 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference antagonist for APLNR
PDF