ADRB2 cAMP Antagonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2064
GPCR Name
Human beta-2 adrenergic receptor
Gene Name
ADRB2
GPCR Sub Family
Adrenoceptors
Coupling Partner
Gαs
Uniprot Number
P07550 (https://www.uniprot.org/uniprotkb/P07550/entry)
Cell Type
HEK293/β-arrestin-2 stable cell line
Assay Information
Assay Principle
The cAMP antagonist assay detects antagonist-mediated inhibition of cAMP production via activation of transiently transfected ADRB2 in HEK293/β-arrestin-2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Antagonist
Assay Readout
FRET fluorescence
Reference Compound EC50
| Compound | EC50 (nM) |
|---|---|
| (S)-Propranolol | 36.1 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference antagonist for ADRB2
PDF