Neurokinin NK3 Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
NK3 Binding
GPCR Target
Human NK3
Source
Membrane preparation from CHO-K1 cells stably transfected with human NK3 receptor.
Scientific Information
NK3R (https://www.uniprot.org/uniprotkb/P29371/entry)
Assay Properties
Protocol
Radioligand filter binding to NK3 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter.
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
SB-222200, 1.5 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| SB-222200 | 4.2 |
| Neurokinin B | 151 |
| Senktide | 643 |
| Osanetant | 5.7 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for NK3
PDF