CDK8/Cyclin C Kinase Assay Service

Activity-based CDK8/Cyclin C kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

CDK8/Cyclin C

Enzyme

Human CDK8 (full length); Cyclin C (full length); MED12 (aa 1-100) complex

HGNC

CDK8

Synonym

CDK8: Cyclin-dependent kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35
Cyclin C: SRB11 homolog (hSRB11), CCNE
MED12: ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230

Scientific Information

CDK8: P49336 (https://www.uniprot.org/uniprot/P49336)
Cyclin C: P24863 (http://www.uniprot.org/uniprot/P24863)
MED12: Q93074 (http://www.uniprot.org/uniprot/Q93074)

Assay Properties
Substrates

Protein substrate, Pol2-CTD, 5 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 83
BI-1347 3.9
BRD6989 11.9
CCT251921 8.3
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Screening Assay Services

Reference compound inhibition for CDK8/Cyclin C

Reference compound inhibition for CDK8/Cyclin C

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