ADRA1A Functional Cell-based Antagonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
ADRA1A Functional Antagonist
GPCR Target
Human ADRA1A
Cell Line
CHO-K1/ADRA1A
Scientific Information
ADA1A (https://www.uniprot.org/uniprotkb/P35348/entry)
Assay Properties
Reaction
30 min incubation with antagonist; ADRA1A activation by agonist application
using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence
kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| Prazosin | 3.09 |
| Alfuzosin | 54.2 |
| Silodosin | 191 |
| Agonist | (nM) |
|---|---|
| Epinephrine | 20 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound inhibition of ADRA1A
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