M1 β-arrestin Agonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2016
GPCR Name
Muscarinic acetylcholine receptor M1
Gene Name
CHRM1
GPCR Sub Family
Acetylcholine receptors (muscarinic)
Coupling Partner
β-arrestin-1&2
Uniprot Number
P11229 (https://www.uniprot.org/uniprotkb/P11229/entry)
Cell Type
U2OS/M1/β-arrestin-1&2 stable cell line
Assay Information
Assay Principle
The LinkLight™ β-arrestin agonist assay measures agonist-induced recruitment of β-arrestin-1&2 via M1 activation in U2OS/M1/β-arrestin-1&2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Agonist
Assay Readout
Luminescence
Reference Compound EC50
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference agonist for M1
