Adenosine A2A Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
A2A Binding
GPCR Target
Human A2A
Source
Membrane preparation from HEK-293 cells stably transfected with human Adenosine A2A receptor.
Scientific Information
ADORA2A (https://www.uniprot.org/uniprotkb/C9J0Z4/entry)
Assay Properties
Protocol
Radioligand filter binding to A2A membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter.
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
CGS21680, 15 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| CGS21680 | 143 |
| NECA | 74 |
| DPCPX | 46 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for A2A
PDF