Activity-based AAK1 kinase assay for compound screening and profiling via Probe-Displacement kinase assay.
Target Overview
CAT#
AAK1 (Binding)
Enzyme
Human AAK1 (27-365)
HGNC
AAK1
Synonym
AP2-associated protein kinase 1, KIAA1048, Adaptor-associated kinase 1
Assay Properties
Probe
Staurosporine-Red, 20 nM
Assay Format
Binding interaction between Staurosporine-Red and Kinase is quantified by HTRF technology using anti Tag HTRF reagents. Compounds binding in this binding pocket displace fluorescently labeled probe resulting loss of binding signal.
Readout
HTRF Readout, Probe-Displacement
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 0.6 nM |
| Dasatinib | 237 nM |
| PP2 | 1.2 µM |
| Sunitinib | 5.6 nM |
Scientific Information
Q2M2I8 (http://www.uniprot.org/uniprot/Q2M2I8)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of AAK1
