Activity-based AAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AAK1
Enzyme
Human AAK1 (27-365)
HGNC
AAK1
Synonym
AP2-associated protein kinase 1, KIAA1048, Adaptor-associated kinase 1
Scientific Information
Q2M2I8 (http://www.uniprot.org/uniprot/Q2M2I8)
Assay Properties
Substrates
AP2M1tide (SQITSQVTGQIGWRREG), 200 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of AAK1
