ABL1 (Y245F/Y412F) Kinase Assay Service

Activity-based ABL1 (Y245F/Y412F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

ABL1 (Y245F/Y412F)

Enzyme

Human ABL1 (Y245F/Y412F)

HGNC

ABL1 (Y245F/Y412F)

Synonym

Proto-oncogene tyrosine-protein kinase ABL1, c-ABL, p150, Abelson murine leukemia viral oncogene homolog 1

Scientific Information

P00519 (http://www.uniprot.org/uniprot/P00519)

Assay Properties
Substrates

Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 59
PP2 160
AG1478 320
EGFR/Erb Inhibitor >100,000
D-64406 190
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for ABL1 (Y245F/Y412F)