ABL1 Y253F Kinase Activity Assay Service

Activity-based ABL1 Y253F kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

ABL1 Y253F

Enzyme

Human ABL1 Y253F, mutant

HGNC

ABL1 (Y253F)

Manning ID

ABL1

Synonym

ABL1, ABL, JTK7, p150, c-ABL, v-abl, ABL1 Y253F, ABL1 (Y253F)

UniProt

P00519 (ABL1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP

Reaction

Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 800
Sorafenib 5600
Sunitinb 8500

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice ABL1 Y253F
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 ABL1 Profiler
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – ABL1 Y253F
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0927-0000-1
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