ACK1 Kinase Assay Service

Activity-based ACK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ACK1
Enzyme
Human ACK1
HGNC
TNK2
Synonym
ACK, p21cdc42Hs, Activated CDC42 kinase 1
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine22.8
PP2220
AG1478220
PDGFR I-III19,000
D-6440613,000
Scientific Information
Q07912 (http://www.uniprot.org/uniprot/Q07912)
Screening Location
Malvern, USA
Reference compound IC50 for ACK1/TNK2
Reference compound IC50 for ACK1/TNK2