ADRA1A IP1 Accumulation Antagonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2058
GPCR Name
Human Alpha-1A adrenergic receptor
Gene Name
ADRA1A
GPCR Sub Family
Adrenoceptors
Coupling Partner
Gαq
Uniprot Number
P35348 (https://www.uniprot.org/uniprotkb/P35348/entry)
Cell Type
U2OS/ADRA1A/β-arrestin-1&2 stable cell line
Assay Information
Assay Principle
The IP1 accumulation antagonist assay measures antagonist-mediated inhibition of agonist-induced IP1 accumulation via ADRA1A activation in U2OS/ADRA1A/β-arrestin-1&2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Antagonist
Assay Readout
Fluorescence
Reference Compound EC50
| Compound | EC50 (nM) |
|---|---|
| WB4101 | 2.31 |
| Prazosin | 5.03 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference antagonist for ADRA1A
PDF