ADRA2A cAMP Antagonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2060
GPCR Name
Human Alpha-2A adrenergic receptor
Gene Name
ADRA2A
GPCR Sub Family
Adrenoceptors
Coupling Partner
Gαi
Uniprot Number
P08913 (https://www.uniprot.org/uniprotkb/P08913/entry)
Cell Type
U2OS/ADRA2A/β-arrestin-2 stable cell line
Assay Information
Assay Principle
The cAMP antagonist assay detects antagonist-mediated promotion of cAMP production via ADRA2A activation in U2OS/ADRA2A/β-arrestin-2 stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Antagonist
Assay Readout
FRET fluorescence
Reference Compound EC50
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference antagonist for ADRA2A
