Activity-based AKT1 E17K kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
AKT1 E17K
Enzyme
Human AKT1, E17K mutant
HGNC
AKT1
Manning ID
AKT1
Synonym
PKB, RAC, PRKBA, RAc-ALPHA
UniProt
P31749 (AKT1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3, ATP
Reaction
RBER-GSK3 + 33P-ATP = 33P-RBER-GSK3 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 90 |
| Sorafenib | >100000 |
| Sunitinib | 50000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | AKT1 E17K |
| wt Profiler Panel | No |
| IC50 wt Profiler Panel | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – AKT1 E17K (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 2261-0000-2 |
| Web Shop | Reaction Biology Online Shop |
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