AKT1 aa106-480 Kinase Activity Assay Service

Activity-based AKT1 aa106-480 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

AKT1 aa106-480

Enzyme

Human AKT1 aa106-480, wt

HGNC

AKT1 (aa106-480)

Manning ID

AKT1

Synonym

PKB, RAC, PRKBA, RAC-ALPHA, AKT1, AKT1 aa106-480

UniProt

P31749 (AKT1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

AGC

Assay Properties

Assay Format

33PanQinaseTM

Substrates

RBER-GSK3(14-27), ATP

Reaction

RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 100
Sorafenib 10000
Sunitinb 52000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice AKT1 aa106-480
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – AKT1 aa106-480
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0132-0000-2
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