AKT2 (E17K) Kinase Assay Service

Activity-based AKT2 (E17K) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AKT2 (E17K)
Enzyme
Human AKT2 (E17K)
HGNC
AKT2 (E17K)
Synonym
PRKBB, PKBBETA, RAc-BETA
Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine3.7
Ro 31-8220250
GW 5074>50,000
H-89830
Rottlerin>50,000
Scientific Information
P31751 (http://www.uniprot.org/uniprot/P31751)
Screening Location
Malvern, USA
Reference compound IC50 for AKT2 (E17K)
Reference compound IC50 for AKT2 (E17K)