AKT3 (E17K) Kinase Assay Service

Activity-based AKT3 (E17K) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AKT3 (E17K)
Enzyme
Human AKT3 (E17K)
HGNC
AKT3 (E17K)
Synonym
PKBG, PKB gamma, PRKBG, RAC-gamma, RAC-PK-gamma, STK-2
Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q9Y243 (http://www.uniprot.org/uniprot/Q9Y243)
Screening Location
Malvern, USA
Reference compound IC50 for AKT3 (E17K)