AKT3 Kinase Activity Assay Service

Activity-based AKT3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

AKT3

Enzyme

Human AKT3, wt

HGNC

AKT3

Manning ID

AKT3

Synonym

PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, AKT3, PKB gamma, DKFZp434N0250, AKT3 aa1-479

UniProt

Q9Y243 (AKT3_HUMAN)

Kinase Type

Protein kinase

Kinase Group

AGC

Assay Properties
Assay Format

33PanQinaseTM

Substrates

RBER-GSK3(14-27), ATP

Reaction

RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 180
Sorafenib 10000
Sunitinib 1700
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice AKT3
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – AKT3
(Activity verified by 33PanQinaseTM-Assay)
Product Number 1578-0000-1
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