ALK-KIF5B (Kex24Aex20) Kinase Assay Service

Activity-based ALK-KIF5B (Kex24Aex20) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ALK, KIF5B (Kex24Aex20) –
Enzyme
Human KIF5B -ALK (Kex24Aex20) fusion
HGNC
ALK-KIF5B
Synonym
CD246, NBLST3
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
KIF5B: P33176 (http://www.uniprot.org/uniprot/P33176)ALK; Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)
Screening Location
Malvern, USA
Reference compound IC50 for ALK-KIF5B (Kex24Aex20)