ALK-NPM1 Kinase Assay Service

Activity-based ALK-NPM1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ALK, NPM1-
Enzyme
Human NPM1-ALK fusion
HGNC
ALK-NPM1
Synonym
CD246, NBLST3
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
NPM1; P06748 (http://www.uniprot.org/uniprot/P06748)ALK; Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)
Screening Location
Malvern, USA
Reference compound IC50 for ALK-NPM1