ALK (F1174L)-EML4 Kinase Assay Service

Activity-based ALK (F1174L)-EML4 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ALK (F1174L), EML4-

Enzyme

Human EML4-ALK (F1174L) fusion

HGNC

ALK (F1174L)-EML4

Synonym

CD246, NBLST3

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

A6P4T4 (http://www.uniprot.org/uniprot/A6P4T4)

Screening Location

Malvern, USA

Reference compound IC50 for ALK (F1174L)-EML4