ALK F1174S Kinase Activity Assay Service

Activity-based ALK F1174S kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

ALK F1174S

Enzyme

Human ALK F1174S, mutant

HGNC

ALK (F1174S)

Manning ID

ALK

Synonym

ALK, CD246, NBLST3, ALK F1174S (GST-HIS-tag), ALK F1174S, ALK (F1174S)

UniProt

Q9UM73 (ALK_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 50
Sorafenib 10000
Sunitinb 340

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice ALK F1174S
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 ALK Profiler
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – ALK F1174S
(Activity verified by 33PanQinaseTM-Assay)
Product Number 1179-0000-1
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