Activity-based ALK R1275Q kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ALK R1275Q
Enzyme
Human ALK R1275Q, mutant
HGNC
ALK (R1275Q)
Manning ID
ALK
Synonym
ALK, CD246, NBLST3, ALK R1275Q (GST-HIS-tag), ALK R1275Q, ALK (R1275Q)
UniProt
Q9UM73 (ALK_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 43 |
| Sorafenib | 7200 |
| Sunitinb | 320 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ALK R1275Q |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 ALK Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ALK R1275Q (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1181-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF