AMPKalpha1 Kinase Activity Assay Service

Activity-based AMPKalpha1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

AMPKalpha1

Enzyme

Human AMPKalpha1, wt

HGNC

PRKAA1

Manning ID

AMPKa1

Synonym

MGC33776, MGC57364, PRKAA1, AMPK-alpha1 aa1-550, AMPKalpha1

UniProt

Q13131 (AAPK1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CAMK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

RBER-CHKtide, ATP

Reaction

RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 16
Sorafenib 8400
Sunitinb 160

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice AMPKalpha1
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – AMPKalpha1
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0289-0000-1

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