APLNR cAMP Agonist Assay for compound screening and profiling.
Target Information
Cell Catalog#
LLC-2094
GPCR Name
Human Apelin receptor
Gene Name
APLNR
GPCR Sub Family
Apelin receptors
Coupling Partner
Gαi
Uniprot Number
P35414 (https://www.uniprot.org/uniprotkb/P35414/entry)
Cell Type
U2OS/APLNR/β-arrestin-1&2 stable cell line
Assay Information
Assay Principle
The cAMP agonist assay detects agonist-induced inhibition of cAMP production via APLNR activation in U2OS/APLNR/β-arrestin stable cell line.
Assay Type
Cell-based functional assay
Functional Mode
Agonist
Assay Readout
Fluorescence
Reference Compound EC50
| Compound | EC50 (nM) |
|---|---|
| Apelin-13 | 0.19 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Representative dose–response curve of reference agonist for APLNR
PDF