ARK5/NUAK1 Kinase Assay Service

Activity-based ARK5/NUAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ARK5/NUAK1
Enzyme
Human ARK5/NUAK1
HGNC
NUAK1
Synonym
KIAA0537, ARK5
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine<0.5
Ro 31-8220270
GW 5074770
H-89220
Scientific Information
O60285 (http://www.uniprot.org/uniprot/O60285)
Screening Location
Malvern, USA
Reference compound IC50 for ARK5/NUAK1
Reference compound IC50 for ARK5/NUAK1