ASH1L Bromodomain Assay Service (ThermoShift)

ASH1L-[BRD]-(GST) Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-Ash1L-[BRD]-GST

Protein

Human ASH1L bromodomain, GST-tagged

Synonyms

ASH-1 like protein; huASH1; KMT2H

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.4 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

47,5

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

Q9NR48 (http://www.uniprot.org/uniprot/Q9NR48)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak