ASH1L Methyltransferase Assay Service

Activity-based ASH1L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
ASH1L
Enzyme
Human ASH1L
Synonym
Histone-lysine N-methyltransferase ASH1L, ASH1-like protein, Lysine N-methyltransferase 2H, Absent small and homeotic disks protein 1 homolog, KMT2H, KIAA1420
Substrates
Oligoucleosomes, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH70,000
Chaetocin52
Sinefungin9,100
Suramin390
Scientific Information
Q9NR48 (http://www.uniprot.org/uniprot/O9NR48)
Screening Location
Malvern, USA
Reference compound IC50 for ASH1L
Reference compound IC50 for ASH1L