Aurora B (G160L) Kinase Assay Service

Activity-based Aurora B (G160L) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
Aurora B (G160L)
Enzyme
Human Aurora B (G160L)
HGNC
AURKB (G160L)
Synonym
AurB, AIK2, AIM1, ARK2, AurB, IPL1, STK5,
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Q96GD4 (http://www.uniprot.org/uniprot/Q96GD4)
Screening Location
Malvern, USA
Reference compound IC50 for Aurora B (G160L)