Activity-based AXL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AXL
Enzyme
Human AXL
HGNC
AXL
Synonym
Tyrosine-protein kinase receptor UFO, AXL oncogene, UFO, EC
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.9 |
| PP2 | 33,000 |
| AG1478 | 2,200 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 4,400 |
Scientific Information
P30530 (http://www.uniprot.org/uniprot/P30530)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for AXL
