BAZ1B Bromodomain Assay Service (ThermoShift)

BAZ1B-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview

CAT#

Tm-BAZ1B-His

Protein

Human BAZ1B bromodomain, His-tagged

Synonyms

hWALp2; WSTF

Assay Properties

Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (5.9 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

44,5

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

Q9UIG0 (http://www.uniprot.org/uniprot/Q9UIG0)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak