BAZ2A Bromodomain Assay Service (ThermoShift)

BAZ2A-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-BAZ2A-GST

Protein

Human BAZ2A bromodomain, GST-tagged

Synonyms

hWALp3; TTF-I-interacting protein 5; Tip5

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.5 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

37,5

Reference Compound ΔTms:

GSK2801 - 5.5 (red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208 and PFI3)

Scientific Information

Q9UIF9 (http://www.uniprot.org/uniprot/Q9UIF9)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak