BAZ2B Bromodomain Assay Service (ThermoShift)

BAZ2B-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human BAZ2B bromodomain, his-tagged


Bromodomain adjacent to zinc finger domain protein 2B; hWALp4; KIAA1476

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (6.2 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

GSK2801 – 3.5 (red)No stabilization was observed in the presence of additional control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208 and PFI3)

Scientific Information

Q9UIF8 (

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak